rt-pcr

网络逆转录-聚合酶链反应; 逆转录聚合酶链式反应; 逆转录-聚合酶链式反应; 反转录聚合酶链反应; 聚合酶链反应

双语例句

The standard testing technique for Ebola is known as reverse transcription polymerase chain reaction, or RT-PCR. 标准的埃博拉检测技术被称作逆转录聚合酶链反应，简称RT-PCR。

But with them, RT-PCR should confirm the virus'presence in about two hours. 但是有了这些引物，RT-PCR应该可以在约2小时内证实这种病毒的存在。

Expression of HCV in Hepatocellular Carcinoma Detected by PCR Technique 应用RT-PCR法检测肝细胞癌的HCV表达

Western blots and RT-PCR were used for protein and gene expression assays. 应用Western蛋白质印记及RT-PCR检测蛋白质及基因表达。

The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blot. 采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。

A multiplex RT-PCR was optimized to simultaneously detect foot-and-mouth disease virus ( FMDV), swine vesicular disease virus ( SVDV) and vesicular stomatitis virus ( VSV). 建立了一种同时检测猪口蹄疫病毒（FMDV）、猪水泡病病毒（SVDV）和猪水疱性口炎病毒（VSV）三种病原体的多重RT-PCR方法。

Research on Exogenous Gene mRNA Levels in Zymomonas mobilis by Semi-quantitative RT-PCR 半定量RT-PCR检测运动发酵单胞菌中外源基因转录水平的研究

METHODS: The expression of MMP-2 in of the pulp of the healthy and carious tooth were examined by immunohistochemical staining, RT-PCR, real-time PCR and western-blot. 以健康、浅龋和深龋牙齿中牙髓组织为研究对象，利用免疫组化、RT-PCR、实时荧光定量PCR和Western印迹检测MMP-2在其中的表达差异。

Establishment of a real-time fluorescent quantitative RT-PCR assay for detection of chicken IL-1 β, IL-2 and IL-6 genes 鸡IL-1β和IL-2及IL-6基因实时荧光定量RT-PCR检测方法的建立

Expression of SHH and PTCH mRNA in esophageal squamous carcinoma by RT-PCR RT-PCR法检测SHH和PTCH在食管鳞状细胞癌中的表达

The Applied Research on the RT-PCR Nucleic Acid Detection and ELISA Antigen Detection Kit of CSFV 猪瘟病毒RT-PCR核酸检测与ELISA抗原检测试验应用研究

RT-PCR ***ysis revealed that the gene is expressed in all embryonic tissues detected. RT-PCR分析表明，该基因在所有被检测的人类胚胎组织中表达。

Detection of Impatiens necrotic spot tospovirus ( INSV) by RT-PCR and Nested PCR 凤仙花坏死斑病毒的RT-PCR和巢式PCR检测

Then extract total RNA from lymphocytes and immediately do the RT-PCR reaction. 从淋巴细胞中提取RNA，然后进行逆转录-聚合酶链（RT-PCR）反应。

RNA from whole blood samples plainly frozen at-20 ℃ can be used in RT-PCR. 简易冻存的外周血标本中提取的RNA，可用于RTPCR实验。

Investigation of gene expression and gene alternative splicing by CE was earned out in this study by ***yzing the RT-PCR products of the target gene, FMR1 gene, a gene leading to fragile X syndrome. 在分子生物学基础研究领域，通过对特定基因的RT-PCR产物的分析，实现了对基因的表达和选择性剪接的直接分析。

Verification of Colorectal Cancer and Liver Metastasis Related Genes Using RT-PCR Method 运用RT-PCR方法对结直肠癌及肝转移相关基因进一步的验证研究

Detection of Chrysanthemum virus B and Tomato aspermy virus by double RT-PCR 两重RT-PCR同步检测菊花B病毒和番茄不孕病毒

The establishment and application of a double PCR for bovine coronavirus and bovine rotavirus 牛冠状病毒和轮状病毒的双重RT-PCR检测方法的建立及应用

Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. 这里，我们使用定向RT-PCR克隆和DNA合成概述了我们最新的进展。

By means of real-time RT-PCR, microporous filters with various nominal pores and materials were compared; 通过实时定量RT-PCR检测，比较了不同材料和不同孔径的微孔滤膜对病毒的吸附效果；

These RT-PCR products were sequence verified. 这些RT-PCR扩增产物的序列验证。

Sugarcane Fiji disease of exotic sugarcane varieties were tested by RT-PCR, and it may further improve the detection techniques of the disease. 应用RT-PCR技术对国外引进的甘蔗品种（材料）进行了甘蔗斐济病的检测，进一步改进和完善了该检测技术。

The detection method for various enteroviruses in water sample by RTPCR with universal primers was established. 建立了一种利用通用引物RT-PCR技术检测水中肠道病毒的方法。

Simultaneous detection of enterovirus 71 and coxsackievirus A16 by multiplex real-time PCR with an internal control 内标多重荧光RT-PCR同时检测肠道病毒71型和柯萨奇病毒A16型

Objective To develop a simple and accurate method for the quantification of real-time RT-PCR gene products. 目的利用实时RT-PCR技术建立一种简便、准确的基因表达相对定量方法。

Optimization of Methods for Total RNA Extraction from Chicken Adipose and RT-PCR Detection 鸡脂肪组织总RNA提取方法的优化和RT-PCR检测

The result of RT-PCR showed that the gene expressed in all stages and tissues of Bombyx mori. PCR检测表明该基因在所调查的家蚕发育时期和组织中均有表达。

Real-time RT-PCR and western blotting were used to assess the relative adiponectin receptor mRNA expression and protein production, respectively. 用荧光定量逆转录PCR和免疫蛋白印迹评估了相关脂联素受体各自的基因表达和蛋白产生。